Isolation and Characterization of TGF-beta 2 and TGF-beta 5 from Medium Conditioned by Xenopus XTC Cells

被引：30

作者：

Roberts, Anita B. ^{[1
]}

Rosa, Frederic ^{[2
]}

Roche, Nanette S. ^{[1
]}

Coligan, John E. ^{[3
]}

Garfield, Mark ^{[3
]}

Rebbert, Martha L. ^{[2
]}

Kondaiah, Paturu ^{[1
]}

Danielpour, David ^{[1
]}

Kehrl, John H. ^{[4
]}

Wahl, Sharon M. ^{[5
]}

Dawid, Igor B. ^{[2
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NCI, Lab Chemoprevent, Bldg 41,Room C629, Bethesda, MD 20892 USA

[2] NICHHD, Lab Mol Genet, Bethesda, MD 20892 USA

[3] NIAID, Biol Resources Branch, Bethesda, MD 20892 USA

[4] NIAID, Lab Immunoregulat, Bethesda, MD 20892 USA

[5] NIDR, Lab Microbiol & Immunol, NIH, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 2卷 / 02期

关键词：

TGF-beta; Xenopus laevis; mesoderm induction; HPLC;

D O I：

10.3109/08977199009071500

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

TGF-beta 2 and -beta 5 have been purified from medium conditioned by Xenopus cultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations, Xenopus TGF-beta 2, like porcine TGF-beta 2, induces expression of mesodermal markers from cultured Xenopus ectodermal explants, whereas TGF-beta 5 is inactive in this assay. However, the TGF-beta's scould be separated from the major mesoderm-inducing activity present in XTC medium. Xetiopus TGF-beta 2 and -beta 5 are approximately equivalent to TGF-beta 1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-beta 5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-beta s.

引用

页码：135 / 147

页数：13

共 40 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] THE TRANSFORMING GROWTH-FACTOR-BETA SYSTEM, A COMPLEX PATTERN OF CROSS-REACTIVE LIGANDS AND RECEPTORS [J].

CHEIFETZ, S ;

WEATHERBEE, JA ;

TSANG, MLS ;

ANDERSON, JK ;

MOLE, JE ;

LUCAS, R ;

MASSAGUE, J .

CELL, 1987, 48 (03) :409-415

[3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[4] IMMUNODETECTION AND QUANTITATION OF THE 2 FORMS OF TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA-1 AND TGF-BETA-2) SECRETED BY CELLS IN CULTURE [J].

DANIELPOUR, D ;

DART, LL ;

FLANDERS, KC ;

ROBERTS, AB ;

SPORN, MB .

JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 138 (01) :79-86

[5]

Danielpour D., 1989, GROWTH FACTORS

[6]

Dawid I. B., 1989, CURR TOPICS DEV BIOL

[7] COMPLEMENTARY-DNA FOR HUMAN GLIOBLASTOMA-DERIVED T-CELL SUPPRESSOR FACTOR, A NOVEL MEMBER OF THE TRANSFORMING GROWTH FACTO-BETA GENE FAMILY [J].

DEMARTIN, R ;

HAENDLER, B ;

HOFERWARBINEK, R ;

GAUGITSCH, H ;

WRANN, M ;

SCHLUSENER, H ;

SEIFERT, JM ;

BODMER, S ;

FONTANA, A ;

HOFER, E .

EMBO JOURNAL, 1987, 6 (12) :3673-3677

[8] A NEW TYPE OF TRANSFORMING GROWTH FACTOR-BETA, TGF-BETA-3 [J].

DERYNCK, R ;

LINDQUIST, PB ;

LEE, A ;

WEN, D ;

TAMM, J ;

GRAYCAR, JL ;

RHEE, L ;

MASON, AJ ;

MILLER, DA ;

COFFEY, RJ ;

MOSES, HL ;

CHEN, EY .

EMBO JOURNAL, 1988, 7 (12) :3737-3743

[9] AMINO-ACID SEQUENCE OF THE BSC-1 CELL-GROWTH INHIBITOR (POLYERGIN) DEDUCED FROM THE NUCLEOTIDE-SEQUENCE OF THE CDNA [J].

HANKS, SK ;

ARMOUR, R ;

BALDWIN, JH ;

MALDONADO, F ;

SPIESS, J ;

HOLLEY, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :79-82

[10] ROLE OF TRANSFORMING GROWTH FACTOR-BETA IN THE DEVELOPMENT OF THE MOUSE EMBRYO [J].

HEINE, UI ;

MUNOZ, EF ;

FLANDERS, KC ;

ELLINGSWORTH, LR ;

LAM, HYP ;

THOMPSON, NL ;

ROBERTS, AB ;

SPORN, MB .

JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2861-2876

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