IDENTIFICATION OF G-PROTEIN-REGULATED INWARDLY RECTIFYING K+ CHANNELS IN RAT-BRAIN OLIGODENDROCYTES

被引：18

作者：

KARSCHIN, A

WISCHMEYER, E

机构：

[1] Max-Planck-Institute for Biophysical Chemistry, Molecular Neurobiology of Signal Transduction

来源：

NEUROSCIENCE LETTERS | 1995年 / 183卷 / 1-2期

关键词：

OLIGODENDROGLIA; K+ CHANNELS; INWARD RECTIFICATION; G PROTEIN REGULATION; MESSENGER-RNA AMPLIFICATION; POLYMERASE CHAIN REACTION; IRK1; ROMK1;

D O I：

10.1016/0304-3940(94)11133-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Rat brain oligodendroglia in culture express a dominant inwardly rectifying K+ current I-KIR which can be inhibited through G. proteins by the activation of glial G protein-coupled receptors. Electrophysiologically we have isolated in these cells several conductances of K+ inward rectifiers (Km) between 12 and 175 pS. Experiments on the single channel level with preloading or bath-application of GTPyS revealed the selective suppression of an 18 pS and maybe other K-IR conductances, possibly via a direct membrane-delimited mechanism. mRNA amplification from single oligodendrocytes together with polymerase chain reaction resulted in the isolation of IRK-type K+ channels which may correspond to the channel species negatively controlled by G proteins.

引用

页码：135 / 138

页数：4