HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION AND ELECTROCHEMICAL DETECTION OF PENICILLINS

被引：25
|
作者：
KIRCHMANN, E [1 ]
WELCH, LE [1 ]
机构：
[1] KNOX COLL, DEPT MED, GALESBURG, IL 61401 USA
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 633卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(93)83144-H
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A reversed-phase high-performance liquid chromatography method used to determine penicillins is described. A C18 stationary phase is used in conjunction with mixed solvent systems containing acetate buffer, methanol, and acetonitrile in various proportions. An isocratic separation is illustrated, but usage of a gradient program varying the composition of the organic modifier gives better performance. Detection is accomplished using pulsed amperometric detection to indirectly monitor the penicillins. The detection limit for ampicillin is 4 . 10(-7) M.
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页码：111 / 118
页数：8
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