CHROMOSOMAL STRUCTURE AND EXPRESSION OF THE HUMAN OTF1 LOCUS ENCODING THE OCT-1 PROTEIN

被引：31
作者：
STURM, RA
CASSADY, JL
DAS, G
ROMO, A
EVANS, GA
机构：
[1] SALK INST BIOL STUDIES, MOLEC GENET LAB, LA JOLLA, CA 92037 USA
[2] COLD SPRING HARBOR LAB, COLD SPRING HARBOR, NY 11724 USA
来源：
GENOMICS | 1993年 / 16卷 / 02期
关键词：
D O I：
10.1006/geno.1993.1194
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The genomic structure of the POU domain containing oct-1 gene (OTF1 locus) coding region has been determined using human DNA recombinant bacteriophage and a yeast artificial chromosome clone. The gene is encoded by 16 exons spanning over 150 kb, and the Oct-1 protein reading frame has been extended to 766 amino acids. The exonic structure has been compared to the mouse Oct-2 protein and reveals a conservation of exon-intron boundaries as well as protein sequence similarity. To provide insight into Oct-1 control of transcriptional regulation during the cell-cycle the expression of the oct -1 gene was examined during cellular DNA replication and shows that the steady-state level of the oct-1 mRNA is not S-phase regulated. © 1993 Academic Press, Inc.
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页码：333 / 341
页数：9
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