SPLITTING OF CIS-SYN CYCLOBUTANE THYMINE-THYMINE DIMERS BY RADIOLYSIS AND ITS RELEVANCE TO ENZYMATIC PHOTOREACTIVATION

被引：40
作者：
HEELIS, PF [1 ]
DEEBLE, DJ [1 ]
KIM, ST [1 ]
SANCAR, A [1 ]
机构：
[1] UNIV N CAROLINA,DEPT BIOCHEM,CHAPEL HILL,NC 27599
来源：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1992年 / 62卷 / 02期
关键词：
D O I：
10.1080/09553009214551941
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Cs-137-gamma-irradiation of cis-syn thymine-thymine cyclobutane type dimers has been studied in aqueous solution. The mechanism of thymine dimer cleavage by e(aq)-, CO2.-, OH.,SO4.-, Br2.-, and isopropanol radicals was studied using high pressure liquid chromatography (HPLC). Evidence that the one-electron reductants studied induce dimer cleavage partially by a chain reaction is presented. Approximate values for the one-electron reduction potential of thymine-thymine are obtained and thermodynamic calculations are presented in order to predict the direction of electron transfer in the case of enzymatic photoreactivation.
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页码：137 / 143
页数：7
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