NANOSCALE SEPARATIONS - CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND CAPILLARY ZONE ELECTROPHORESIS MASS-SPECTROMETRY FOR THE DETERMINATION OF PEPTIDES AND PROTEINS

被引：50
作者：
DETERDING, LJ
PARKER, CE
PERKINS, JR
MOSELEY, MA
JORGENSON, JW
TOMER, KB
机构：
[1] NIEHS, MOLEC BIOPHYS LAB, POB 12233, RES TRIANGLE PK, NC 27709 USA
[2] UNIV N CAROLINA, DEPT CHEM, CHAPEL HILL, NC 27514 USA
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 554卷 / 1-2期
关键词：
D O I：
10.1016/S0021-9673(01)88460-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Nanoscale separation techniques, such as packed capillary liquid chromatography and capillary zone electrophoresis in which the column effluent flow-rates are in the nanoliter per minute range rather than microliter per minute range, have been successfully interfaced with continuous-flow fast atom bombardment and electrospray ionization. Applications of these techniques to the separation and determination of peptide mixtures, protein digests and protein mixtures are presented.
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页码：329 / 338
页数：10
相关论文
共 22 条
[1]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - PAST DEVELOPMENTS, PRESENT STATUS, AND FUTURE-TRENDS [J].
BROWN, PR .
ANALYTICAL CHEMISTRY, 1990, 62 (19) :A995-&
[2]   CONTINUOUS-FLOW FAST ATOM - BOMBARDMENT MASS-SPECTROMETRY [J].
CAPRIOLI, RM .
ANALYTICAL CHEMISTRY, 1990, 62 (08) :A477-A487
[3]   COUPLING CAPILLARY ZONE ELECTROPHORESIS AND CONTINUOUS-FLOW FAST ATOM BOMBARDMENT MASS-SPECTROMETRY FOR THE ANALYSIS OF PEPTIDE MIXTURES [J].
CAPRIOLI, RM ;
MOORE, WT ;
MARTIN, M ;
DAGUE, BB ;
WILSON, K ;
MORING, S .
JOURNAL OF CHROMATOGRAPHY, 1989, 480 :247-257
[4]  
CAPRIOLI RM, 1990, CONTINUOUS-FLOW FAST ATOM BOMBARDMENT MASS SPECTROMETRY, P93
[5]  
De Wit JSM, 1988, RAPID COMMUN MASS SP, V2, P100
[6]   NANOSCALE SEPARATIONS COMBINED WITH TANDEM MASS-SPECTROMETRY [J].
DETERDING, LJ ;
MOSELEY, MA ;
TOMER, KB ;
JORGENSEN, JW .
JOURNAL OF CHROMATOGRAPHY, 1991, 554 (1-2) :73-82
[7]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[8]   LC/MS AND LC/MS/MS DETERMINATION OF PROTEIN TRYPTIC DIGESTS [J].
HUANG, EC ;
HENION, JD .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1990, 1 (02) :158-165
[9]   CAPILLARY ZONE ELECTROPHORESIS [J].
JORGENSON, JW ;
LUKACS, KD .
SCIENCE, 1983, 222 (4621) :266-272
[10]  
JORGERSON JW, 1986, ANAL CHEM, V58, pA754
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