IMPROVEMENT OF AXIAL RESOLUTION IN CONFOCAL MICROSCOPY USING AN ANNULAR PUPIL

被引：34
|
作者：
SHEPPARD, CJR
GU, M
机构：
来源：
OPTICS COMMUNICATIONS | 1991年 / 84卷 / 1-2期
关键词：
D O I：
10.1016/0030-4018(91)90019-A
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Reported in this paper arc results on the improvement in axial resolution in scanning confocal microscopy when an annular pupil is combined with a finite-sized pinhole in front of the detector. Theoretical analyses show the optimum relationship of the axial resolution to the pinhole size and the radius of central obstruction. The magnitude of the signal level is also explored. The experimental results are well in agreement with the theoretical predictions.
引用
收藏
页码：7 / 13
页数：7
相关论文
共 50 条
  • [41] Pupil function engineering for confocal microscopy
    Wilson, T
    Massoumian, F
    Juskaitis, R
    LASER BEAM SHAPING IV, 2003, 5175 : 160 - 167
  • [42] Optimal Pupil Design for Confocal Microscopy
    Patel, Yogesh G.
    Rajadhyaksha, Milind
    DiMarzio, Charles A.
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XVII, 2010, 7570
  • [43] Tunable axial resolution in confocal scanning microscopy by controlled symmetrical defocusing
    Martínez-Corral, M
    Kowalczyk, M
    Zapata-Rodríguez, C
    Andrés, P
    OPTICA APPLICATA, 1998, 28 (03) : 213 - 225
  • [44] Improvements of axial resolution in confocal microscopy with fan-shaped apertures
    Ma, Ye
    Kuang, Cuifang
    Gong, Wei
    Xue, Li
    Zheng, Yao
    Wang, Yifan
    Si, Ke
    Liu, Xu
    APPLIED OPTICS, 2015, 54 (06) : 1354 - 1362
  • [45] Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy
    Siegel, Nisan
    Brooker, Gary
    OPTICS EXPRESS, 2014, 22 (19): : 22298 - 22307
  • [46] Confocal microscope with enhanced lateral resolution using engineered illumination pupil
    Boruah, B. R.
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XVII, 2010, 7570
  • [47] Imaging theory and resolution improvement of two-photon confocal microscopy
    唐志列
    杨初平
    裴红津
    梁瑞生
    刘颂豪
    Science China Mathematics, 2002, (11) : 1468 - 1478
  • [48] Imaging theory and resolution improvement of two-photon confocal microscopy
    Tang, ZL
    Yang, CP
    Pei, HJ
    Liang, RS
    Liu, SH
    SCIENCE IN CHINA SERIES A-MATHEMATICS, 2002, 45 (11): : 1468 - 1478
  • [49] Imaging theory and resolution improvement of two-photon confocal microscopy
    Tang Zhilie
    Yang Chuping
    Pei Hongjin
    Liang Ruisheng
    Liu Songhao
    Science in China Series A: Mathematics, 2002, 45 : 1468 - 1478
  • [50] Confocal theta fluorescence microscopy with annular apertures
    Lindek, S
    Cremer, C
    Stelzer, EHK
    APPLIED OPTICS, 1996, 35 (01): : 126 - 130
12345