COMPARATIVE-STUDY OF AUTOMATED VERSUS MANUAL EXTRACTION OF DNA FROM CLINICAL SPECIMENS

被引：7
作者：
TAYLOR, SA
TSANG, AOW
FU, KH
KALOUSEK, DK
HORSMAN, DE
机构：
[1] CANC CONTROL AGCY,DEPT PATHOL,CLIN LABS,600 W 10TH AVE,VANCOUVER V5Z 4E6,BC,CANADA
[2] UNIV BRITISH COLUMBIA,DEPT PATHOL,VANCOUVER V6T 1W5,BC,CANADA
[3] BRITISH COLUMBIA CHILDRENS HOSP,DEPT PATHOL,VANCOUVER,BC,CANADA
来源：
AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1990年 / 93卷 / 06期
关键词：
DNA extraction; Hybridization; Southern transfer;
D O I：
10.1093/ajcp/93.6.749
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The authors have compared an automated method for DNA extraction with an established manual method using routine clinical specimens. The purity, yield, and quality of the extracted DNA was assessed by ultraviolet absorbency measurements, gel electrophoresis, and DNA probe hybridization. The automated DNA extractor can provide high molecular weight DNA equivalent in purity, yield, and quality to that obtained by the manual method with substantial savings in technologist time and overall costs associated with the procedure.
引用
收藏
页码：749 / 753
页数：5
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