THE IMPORTANCE OF EFFECTIVE PREDEVELOPMENT PLATE WASHING TECHNIQUES IN THIN-LAYER CHROMATOGRAPHY

被引：13

作者：

MAXWELL, RJ

YEISLEY, SW

UNRUH, J

机构：

[1] U. S. Department of Agriculture, ARS Eastern Regional Research Center, Philadelphia, Pennsylvania, 19118

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 10期

关键词：

D O I：

10.1080/01483919008049008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative studies were made of the two methods commonly used for predevelopment TLC plate cleaning. In separate studies TLC plates were washed by the two methods: ascending development and dip washing using several solvent and solvent combinations. Residues remaining on the surface of plates after washing were visualized by fluorescence techniques and scanned by densitometry. Evaluation of the two washing methods showed that plate dipping gave superior results in every case over ascending development. The most effective solvent for predevelopment TLC plate washing was found to be methanol. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：2001 / 2011

页数：11

共 7 条

[1] DEVELOPMENT OF RAPID METHODS FOR THE ANALYSIS OF AFLATOXIN IN AGRICULTURAL PRODUCE .1. EVALUATION OF INSTRUMENTATION USED FOR HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY OF AFLATOXINS [J].