HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE AND ITS DERIVATIVES WITH ELECTROCHEMICAL DETECTION

被引：29

作者：

GREENBERG, MS

MAYER, WJ

机构：

[1] Department of Chemistry, Kent State University, Kent

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 169卷 / FEB期

关键词：

D O I：

10.1016/0021-9673(75)85056-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Theophylline and its derivatives are determined by high-performance liquid chromatographic separation on a C8 reversed-phase column with electrochemical detection. The analyte is oxidized at 1.24 V (vs. the standard calomel electrode) on a wax-impregnated graphite electrode using classical DC and current sampled DC modes, and the results obtained compared with those of a 254 nm UV detector. Polarographic detection offers greater sensitivity for theophylline than UV detection; further, interferences which hamper UV detection may be selectively eliminated with polarographic detection. Theophylline (1,3-dimethylxanthine) is used in drug therapy for the control of asthma.

引用

页码：321 / 327

页数：7

共 22 条

[1] DUAL ELECTROCHEMICAL DETECTOR FOR LIQUID-CHROMATOGRAPHY [J].