SPECIFIC TRANSCRIPTIONAL ACTIVATION IN-VITRO BY THE HERPES-SIMPLEX VIRUS PROTEIN VP16

被引：16

作者：

ARNOSTI, DN

PRESTON, CM

HAGMANN, M

SCHAFFNER, W

HOPE, RG

LAUGHLAN, G

LUISI, BF

机构：

[1] UNIV ZURICH,INST MOLEK BIOL 3,CH-8006 ZURICH,SWITZERLAND

[2] MRC,VIROL UNIT,GLASGOW G11 5JR,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 24期

关键词：

D O I：

10.1093/nar/21.24.5570

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcription system. Stimulation of transcription was dependent on the IE-specific element, TAATGARAT. In agreement with earlier observations from electrophoretic mobility shift assays, activation was not observed when Oct-2/POU, the DNA-binding domain from the Oct-2 protein, was substituted for Oct-1/POU. Single round transcription assays revealed that, together, VP16 and Oct-1/POU facilitate the assembly of pre-initiation complexes at target gene promoters.

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页码：5570 / 5576

页数：7

相关论文

共 45 条

[1] MUTATIONAL ANALYSIS OF THE HERPES-SIMPLEX VIRUS TYPE-1 TRANS-INDUCING FACTOR VMW65 [J].

ACE, CI ;

DALRYMPLE, MA ;

RAMSAY, FH ;

PRESTON, VG ;

PRESTON, CM .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :2595-2605

[2] OCT-2 FACILITATES FUNCTIONAL PREINITIATION COMPLEX ASSEMBLY AND IS CONTINUOUSLY REQUIRED AT THE PROMOTER FOR MULTIPLE ROUNDS OF TRANSCRIPTION [J].

ARNOSTI, DN ;

MERINO, A ;

REINBERG, D ;

SCHAFFNER, W .

EMBO JOURNAL, 1993, 12 (01) :157-166

[3] THE SOLUTION STRUCTURE OF THE OCT-1 POU-SPECIFIC DOMAIN REVEALS A STRIKING SIMILARITY TO THE BACTERIOPHAGE-LAMBDA REPRESSOR DNA-BINDING DOMAIN [J].

ASSAMUNT, N ;

MORTISHIRESMITH, RJ ;

AURORA, R ;

HERR, W ;

WRIGHT, PE .

CELL, 1993, 73 (01) :193-205

[4] POU-SPECIFIC DOMAIN OF OCT-2 FACTOR CONFERS OCTAMER MOTIF DNA-BINDING SPECIFICITY ON HETEROLOGOUS ANTENNAPEDIA HOMEODOMAIN [J].

BRUGNERA, E ;

XU, L ;

SCHAFFNER, W ;

ARNOSTI, DN .

FEBS LETTERS, 1992, 314 (03) :361-365

[5] IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION [J].

CAMPBELL, MEM ;

PALFREYMAN, JW ;

PRESTON, CM .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (01) :1-19

[6] DIFFERENTIAL POSITIVE CONTROL BY OCT-1 AND OCT-2 - ACTIVATION OF A TRANSCRIPTIONALLY SILENT MOTIF THROUGH OCT-1 AND VP16 CORECRUITMENT [J].

CLEARY, MA ;

STERN, S ;

TANAKA, M ;

HERR, W .

GENES & DEVELOPMENT, 1993, 7 (01) :72-83

[7] SOLUTION STRUCTURE OF THE POU-SPECIFIC DNA-BINDING DOMAIN OF OCT-1 [J].

DEKKER, N ;

COX, M ;

BOELENS, R ;

VERRIJZER, CP ;

VANDERVLIET, PC ;

KAPTEIN, R .

NATURE, 1993, 362 (6423) :852-855

[8] A HERPESVIRUS TRANS-ACTIVATING PROTEIN INTERACTS WITH TRANSCRIPTION FACTOR OTF-1 AND OTHER CELLULAR PROTEINS [J].

GERSTER, T ;

ROEDER, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6347-6351

[9] SEPARATION OF REQUIREMENTS FOR PROTEIN-DNA COMPLEX ASSEMBLY FROM THOSE FOR FUNCTIONAL-ACTIVITY IN THE HERPES-SIMPLEX VIRUS REGULATORY PROTEIN VMW65 [J].

GREAVES, R ;

OHARE, P .

JOURNAL OF VIROLOGY, 1989, 63 (04) :1641-1650

[10]

HAWLEY D, 1989, J BIOL CHEM, V262, P3452

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