INHIBITION OF REVERSE-TRANSCRIPTASE ACTIVITY BY EXTRACTS OF CULTURED BLUE-GREEN-ALGAE (CYANOPHYTA)

被引：31

作者：

LAU, AF ^{[1
]}

SIEDLECKI, J ^{[1
]}

ANLEITNER, J ^{[1
]}

PATTERSON, GML ^{[1
]}

CAPLAN, FR ^{[1
]}

MOORE, RE ^{[1
]}

机构：

[1] UNIV HAWAII,DEPT CHEM,HONOLULU,HI 96813

来源：

PLANTA MEDICA | 1993年 / 59卷 / 02期

关键词：

BLUE-GREEN ALGAE; REVERSE TRANSCRIPTASE ACTIVITY; HIV; EXTRACTS; INHIBITORS;

D O I：

10.1055/s-2006-959631

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Lipophilic and hydrophilic extracts of over 900 strains of cultured blue-green algae (cyanophyta) were examined in vitro for their ability to inhibit the reverse transcriptases (RT) of avian myeloblastosis virus (AMV) and human immunodeficiency virus, type 1 (HIV-1). Eighteen (2.0 %) aqueous extracts showed activity against AMV and HIV RTs. The maximal level of RT inhibition achieved by some of the active extracts was equivalent to that measured for 3'-azido-2',3'-di-deoxythymidine (AZT) at 668 ng/ml. Examination of partially purified fractions prepared by C18 column chromatography demonstrated that the RT inhibition observed could not be attributed entirely to the degradation of transcript DNA, template RNA, or enzyme protein in the reaction mixture. Thus, these results indicate that cultured blue-green algae may represent a novel source of compounds that inhibit RT activity, including that of HIV-1.

引用

页码：148 / 151

页数：4

共 15 条

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[2]

Fautin DG, 1988, MEM CAL ACAD SCI, P143

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