FUNCTIONAL-CHARACTERIZATION OF RK5, A VOLTAGE-GATED K+ CHANNEL CLONED FROM THE RAT CARDIOVASCULAR-SYSTEM

被引：60
作者：
BLAIR, TA
ROBERDS, SL
TAMKUN, MM
HARTSHORNE, RP
机构：
[1] OREGON HLTH SCI UNIV,DEPT PHARMACOL,PORTLAND,OR 97201
[2] VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOPHYS,NASHVILLE,TN 37232
[3] VANDERBILT UNIV,MED CTR,SCH MED,DEPT PHARMACOL,NASHVILLE,TN 37232
[4] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MOLEC PHYSIOL,NASHVILLE,TN 37232
来源：
FEBS LETTERS | 1991年 / 295卷 / 1-3期
关键词：
ELECTROPHYSIOLOGY; SHAL; TRANSIENT CURRENT; DENDROTOXIN; XENOPUS OOCYTES;
D O I：
10.1016/0014-5793(91)81420-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A voltage-sensitive K+ channel previously cloned from rat heart designated RK5 (rat Kv4.2) (Roberds and Tamkun, 1991, Proc. Natl. Acad. Sci. USA 88, 1798-1802) was functionally characterized in the Xenopus oocyte expression system. RK5 is a homolog of the Drosophila Shal K+ channel. activates with a rise time of 2.8 ms, has a midpoint for activation of -1 mV and rapidly inactivates with time constants of 15 and 60 ms. RK5 is sensitive to 4-AP, IC50 = 5 mM, and is insensitive to TEA and dendrotoxins. The voltage dependence and kinetics of the RK5 induced currents suggest this channel contributes to the I(to) current in heart.
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页码：211 / 213
页数：3
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