PSEUDOMONAS-SOLANACEARUM - PLASMID PJTPS1 MEDIATES A SHIFT FROM THE PATHOGENIC TO NONPATHOGENIC PHENOTYPE

被引：9

作者：

NEGISHI, H ^{[1
]}

YAMADA, T ^{[1
]}

SHIRAISHI, T ^{[1
]}

OKU, H ^{[1
]}

TANAKA, H ^{[1
]}

机构：

[1] OKAYAMA UNIV,COLL AGR,PLANT PATHOL LAB,OKAYAMA 700,JAPAN

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1993年 / 6卷 / 02期

关键词：

NONPATHOGENICITY;

D O I：

10.1094/MPMI-6-203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate the role of a small plasmid, pJTPS1, harbored by a spontaneous nonpathogenic mutant (M4S) of Pseudomonas solanacearum (U-7), a recombinant plasmid pJTPS2, expressing a hygromycin resistance gene inserted in pJTPS1, was transformed into the parental pathogenic strain, U-7. The hygromycin-resistant transformant (U-7T1) formed nonfluidal colonies similar to M4S and lost pathogenicity on tobacco and tomato. Moreover, the amount of extracellular polysaccharide (EPS) production and the level of endoglucanase (EG) activity in U-7T1 were decreased to levels lower than those of M4S. Furthermore, a pJTPS2-cured mutant isolated from U-7T1 formed fluidal colonies with high EPS production and EG activity and regained pathogenicity. These data suggest that pJTPS1 reduces or eliminates pathogenicity of the bacterium upon tobacco and tomato.

引用

页码：203 / 209

页数：7