PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE - FULL-LENGTH PIG BLASTOCYST CDNA SEQUENCE AND REGULATION BY SELENIUM STATUS

被引：61

作者：

SUNDE, RA ^{[1
]}

DYER, JA ^{[1
]}

MORAN, TV ^{[1
]}

EVENSON, JK ^{[1
]}

SUGIMOTO, M ^{[1
]}

机构：

[1] UNIV MISSOURI,COLL AGR FOOD & NAT RESOURCES,DEPT BIOCHEM,COLUMBIA,MO 65211

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1711

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classicial glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase. © 1993 Academic Press, Inc.

引用

页码：905 / 911

页数：7

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