MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE

被引：92

作者：

PARASASSI, T ^{[1
]}

RAVAGNAN, G ^{[1
]}

RUSCH, RM ^{[1
]}

GRATTON, E ^{[1
]}

机构：

[1] UNIV ILLINOIS,FLUORESCENCE DYNAM LAB,URBANA,IL 61801

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 57卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1993.tb02309.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steady-state and dynamic fluorescence properties of 6-lauroyl-2-dimethylaminonaphthalene (Laurdan) have been used to ascertain the coexistence of separate phase domains and their dynamic properties in phospholipid vesicles composed of different mole ratios of dilauroyl- and dipalmitoyl-phosphatidylcholine (DLPC and DPPC, respectively). The recently introduced generalized polarization together with time-resolved emission spectra have been utilized for detecting changes. The results indicate the coexistence of phospholipid phase domains in vesicle compositions in the range between 30 mol% and 70 mol% DPPC in DLPC. Below and above these concentrations a homogeneous phase is observed, with averaged properties. In the case of coexisting phase domains, the properties of each individual phase are largely influenced by the presence of the other phase. Implications on fluctuations between the coexisting phases and on the size and shape of domains are discussed.

引用

页码：403 / 410

页数：8

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