GENOTYPE-INDEPENDENT TRANSFORMATION OF LETTUCE USING AGROBACTERIUM-TUMEFACIENS

被引：76

作者：

CURTIS, IS

POWER, JB

BLACKHALL, NW

DELAAT, AMM

DAVEY, MR

机构：

[1] UNIV NOTTINGHAM,DEPT LIFE SCI,PLANT GENET MANIPULAT GRP,NOTTINGHAM NG7 2RD,ENGLAND

[2] VAN HAVE RES,DEPT BIOTECHNOL,4410 AA RILLAND,NETHERLANDS

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1994年 / 45卷 / 279期

关键词：

AGROBACTERIUM-MEDIATED TRANSFORMATION; LACTUCA SATIVA GENOTYPES; LETTUCE;

D O I：

10.1093/jxb/45.10.1441

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The genetic manipulation of lettuce (Lactuca sativa) necessitates a reliable and efficient, genotype-independent method of transformation. Thirteen lettuce cultivars have been assessed for their amenability to Agrobacterium-mediated gene transfer linked to their tissue culture responsiveness, including callus induction and shoot regeneration. A reliable protocol has been developed for the routine production of transgenic plants for all 13 cultivars investigated.

引用

页码：1441 / 1449

页数：9

共 21 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] TRANSGENIC PLANTS OF LETTUCE (LACTUCA-SATIVA) OBTAINED THROUGH ELECTROPORATION OF PROTOPLASTS [J].