2 ALPHA(1B)-ADRENERGIC RECEPTOR MESSENGER-RNAS EXPRESSED IN SPRAGUE-DAWLEY RAT-LIVER HAVE DISTINCT 5'-REGIONS

被引:7
作者
DENG, CL
CORNETT, LE
机构
[1] UNIV ARKANSAS MED SCI HOSP, DEPT PHYSIOL & BIOPHYS, LITTLE ROCK, AR 72205 USA
[2] UNIV ARKANSAS MED SCI HOSP, DEPT INTERNAL MED, LITTLE ROCK, AR 72205 USA
来源
JOURNAL OF RECEPTOR RESEARCH | 1994年 / 14卷 / 02期
关键词
D O I
10.3109/10799899409067000
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Previous studies have revealed two size classes of alpha(1b)-adrenergic receptor mRNAs, 3.3 kb and 2.7 kb, in the Sprague Dawley rat that are transcribed from a single gene and are expressed in approximately equal amounts in liver. Only the 2.7 kb mRNA is expressed in heart. Both alpha(1b)-adrenergic receptor mRNAs appear to share extensive regions of homology, therefore, we used oligonucleotide-directed ribonuclease H mapping to detect sequence differences between the two transcripts. Initial experiments using oligo (dT)-directed RNase H hydrolysis indicated that the two mRNAs have poly [A(+)] tails of identical length. By using region-specific cDNA probes, we determined that the sequence difference between the two alpha(1b)-adrenergic receptor mRNAs lies in the 5' end, upstream from the known initator AUG in the 2.7 kb transcript. In addition, results from ribonuclease protection assays and Northern blot analysis in which an oligonucleotide was used as the probe suggested that both alpha(1b)-adrenergic receptor mRNAs are transcribed from the same DNA strand.
引用
收藏
页码:119 / 137
页数:19
相关论文
共 30 条
[1]   2 MAMMALIAN GENES TRANSCRIBED FROM OPPOSITE STRANDS OF THE SAME DNA LOCUS [J].
ADELMAN, JP ;
BOND, CT ;
DOUGLASS, J ;
HERBERT, E .
SCIENCE, 1987, 235 (4795) :1514-1517
[3]   SUBTYPES OF ALPHA-1-ADRENERGIC AND ALPHA-2-ADRENERGIC RECEPTORS [J].
BYLUND, DB .
FASEB JOURNAL, 1992, 6 (03) :832-839
[4]   THE VASOPRESSIN MESSENGER-RNA POLY(A) TRACT IS UNUSUALLY LONG AND INCREASES DURING STIMULATION OF VASOPRESSIN GENE-EXPRESSION INVIVO [J].
CARRAZANA, EJ ;
PASIEKA, KB ;
MAJZOUB, JA .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2267-2274
[5]   A METHOD FOR ISOLATION OF INTACT, TRANSLATIONALLY ACTIVE RIBONUCLEIC-ACID [J].
CATHALA, G ;
SAVOURET, JF ;
MENDEZ, B ;
WEST, BL ;
KARIN, M ;
MARTIAL, JA ;
BAXTER, JD .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1983, 2 (04) :329-335
[6]   CAMP STIMULATES TRANSCRIPTION OF THE BETA-2-ADRENERGIC RECEPTOR GENE IN RESPONSE TO SHORT-TERM AGONIST EXPOSURE [J].
COLLINS, S ;
BOUVIER, M ;
BOLANOWSKI, MA ;
CARON, MG ;
LEFKOWITZ, RJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :4853-4857
[7]   MOLECULAR-CLONING AND EXPRESSION OF THE CDNA FOR THE HAMSTER ALPHA-1-ADRENERGIC RECEPTOR [J].
COTECCHIA, S ;
SCHWINN, DA ;
RANDALL, RR ;
LEFKOWITZ, RJ ;
CARON, MG ;
KOBILKA, BK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7159-7163
[8]  
GAO B, IN PRESS GENE
[9]   GENE WITHIN A GENE - NESTED DROSOPHILA GENES ENCODE UNRELATED PROTEINS ON OPPOSITE DNA STRANDS [J].
HENIKOFF, S ;
KEENE, MA ;
FECHTEL, K ;
FRISTROM, JW .
CELL, 1986, 44 (01) :33-42
[10]   TISSUE-SPECIFIC EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-II MESSENGER-RNAS WITH DISTINCT 5' UNTRANSLATED REGIONS [J].
IRMINGER, JC ;
ROSEN, KM ;
HUMBEL, RE ;
VILLAKOMAROFF, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) :6330-6334