A NOVEL RAT SEROTONIN (5-HT6) RECEPTOR - MOLECULAR-CLONING, LOCALIZATION AND STIMULATION OF CAMP ACCUMULATION

被引：349

作者：

RUAT, M ^{[1
]}

TRAIFFORT, E ^{[1
]}

ARRANG, JM ^{[1
]}

TARDIVELLACOMBE, J ^{[1
]}

DIAZ, J ^{[1
]}

LEURS, R ^{[1
]}

SCHWARTZ, JC ^{[1
]}

机构：

[1] UNIV PARIS 05,FAC PHARM,PHYSIOL LAB,F-75270 PARIS 06,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1619

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a strategy based upon nucleotide sequence homology and starting from the sequence of the rat histamine H2 receptor (Ruat et al., Biochem. Biophys. Res. Commun. 1991, 179, 1470-1478), we have cloned a rat cDNA encoding a functional serotonin receptor (5-HT6). Its coding sequence corresponds to a glycoprotein of 436 amino acids displaying significant homology with other cloned monoaminergic receptors, e.g., various serotonin receptors. Genomic analysis of its gene indicated the presence of at least one intron. The major transcript of the 5-HT6 receptor gene has a size of ∼4.1 kb but another minor 3.2 kb transcript was also evidenced. The highest expression, detected by Northern blot analysis as well as by in situ hybridization occurs in various serotoninergic areas of rat or guinea pig brain such as striatum, olfactory tubercle, nucleus accumbens and hippocampus, but a faint expression is also detectable in rat stomach. When transiently expressed in transfected COS-7 cells the 5-HT6 receptor appears to be positively coupled to cyclic AMP production. © 1993 Academic Press, Inc.

引用

页码：268 / 276

页数：9

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