PLATELET-ACTIVATING-FACTOR (PAF) RECEPTOR IN RAT-BRAIN - PAF MOBILIZES INTRACELLULAR CA2+ IN HIPPOCAMPAL-NEURONS

被引：160
作者：
BITO, H
NAKAMURA, M
HONDA, Z
IZUMI, T
IWATSUBO, T
SEYAMA, Y
OGURA, A
KUDO, Y
SHIMIZU, T
机构：
[1] UNIV TOKYO,FAC MED,DEPT NEUROL,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,FAC MED,DEPT PHYSIOL CHEM & NUTR,BUNKYO KU,TOKYO 113,JAPAN
[3] MITSUBISHI KASEI INST LIFE SCI,DEPT NEUROSCI,MACHIDA,TOKYO 194,JAPAN
来源：
NEURON | 1992年 / 9卷 / 02期
关键词：
D O I：
10.1016/0896-6273(92)90167-C
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Platelet-activating factor (PAF), an alkylether phospholipid, is produced in the brain when it is subjected to various stimuli. Using a Xenopus oocyte expression system, we obtained evidence for functional PAF receptor mRNA expression in rat brain. The presence of the PAF receptor was confirmed and shown to be quite ubiquitous in the CNS by RNA blot and radioligand binding studies. To investigate the neuronal functions of PAF, intracellular Ca2+ increase elicited by nanomolar PAF application was analyzed in cultured rat hippocampal cells. Fractions of NMDA-responsive cells and non-NMDA-responsive cells were shown to respond to PAF, suggesting a potential role for PAF in the Ca2+ signaling pathway in the hippocampus.
引用
收藏
页码：285 / 294
页数：10
相关论文
共 56 条
[11]   PLATELET-ACTIVATING FACTOR STIMULATES PROLACTIN-RELEASE FROM DISPERSED RAT ANTERIOR-PITUITARY CELLS-INVITRO [J].
CAMORATTO, AM ;
GRANDISON, L .
ENDOCRINOLOGY, 1989, 124 (03) :1502-1506
[12]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[13]   INHIBITION OF LONG-TERM POTENTIATION BY AN ANTAGONIST OF PLATELET-ACTIVATING-FACTOR RECEPTORS [J].
DELCERRO, S ;
ARAI, A ;
LYNCH, G .
BEHAVIORAL AND NEURAL BIOLOGY, 1990, 54 (03) :213-217
[14]  
DEMOPOULOS CA, 1979, J BIOL CHEM, V254, P9355
[15]   [H-3]WEB-2086 LABELS PLATELET ACTIVATING FACTOR RECEPTORS IN GUINEA-PIG AND HUMAN-LUNG [J].
DENT, G ;
UKENA, D ;
SYBRECHT, GW ;
BARNES, PJ .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 169 (2-3) :313-316
[16]   PRESENCE OF SPECIFIC BINDING-SITES FOR PLATELET-ACTIVATING FACTOR (PAF) IN BRAIN [J].
DOMINGO, MT ;
SPINNEWYN, B ;
CHABRIER, PE ;
BRAQUET, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (02) :730-736
[17]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[18]   THE DENOVO BIOSYNTHESIS OF PLATELET-ACTIVATING FACTOR IN RAT-BRAIN [J].
FRANCESCANGELI, E ;
GORACCI, G .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (01) :107-112
[19]   PLATELET ACTIVATING FACTOR INDUCES INOSITOL PHOSPHATE ACCUMULATION IN CULTURES OF RAT AND BOVINE ANTERIOR-PITUITARY-CELLS [J].
GRANDISON, L .
ENDOCRINOLOGY, 1990, 127 (04) :1786-1791
[20]   PLATELET-ACTIVATING-FACTOR - A BIOLOGICALLY-ACTIVE PHOSPHOGLYCERIDE [J].
HANAHAN, DJ .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :483-509
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