PLATELET-ACTIVATING-FACTOR (PAF) RECEPTOR IN RAT-BRAIN - PAF MOBILIZES INTRACELLULAR CA2+ IN HIPPOCAMPAL-NEURONS

被引：160

作者：

BITO, H

NAKAMURA, M

HONDA, Z

IZUMI, T

IWATSUBO, T

SEYAMA, Y

OGURA, A

KUDO, Y

SHIMIZU, T

机构：

[1] UNIV TOKYO,FAC MED,DEPT NEUROL,BUNKYO KU,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT PHYSIOL CHEM & NUTR,BUNKYO KU,TOKYO 113,JAPAN

[3] MITSUBISHI KASEI INST LIFE SCI,DEPT NEUROSCI,MACHIDA,TOKYO 194,JAPAN

来源：

NEURON | 1992年 / 9卷 / 02期

关键词：

D O I：

10.1016/0896-6273(92)90167-C

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Platelet-activating factor (PAF), an alkylether phospholipid, is produced in the brain when it is subjected to various stimuli. Using a Xenopus oocyte expression system, we obtained evidence for functional PAF receptor mRNA expression in rat brain. The presence of the PAF receptor was confirmed and shown to be quite ubiquitous in the CNS by RNA blot and radioligand binding studies. To investigate the neuronal functions of PAF, intracellular Ca2+ increase elicited by nanomolar PAF application was analyzed in cultured rat hippocampal cells. Fractions of NMDA-responsive cells and non-NMDA-responsive cells were shown to respond to PAF, suggesting a potential role for PAF in the Ca2+ signaling pathway in the hippocampus.

引用

页码：285 / 294

页数：10

共 56 条

[1] RAT HIPPOCAMPAL NEURONS IN DISPERSED CELL-CULTURE [J].