CULTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS FROM WHOLE-BLOOD - EFFECT OF ANTICOAGULANT AND INOCULUM SIZE ON VIRUS GROWTH

被引：11

作者：

BAYLISS, GJ ^{[1
]}

JESSON, WJ ^{[1
]}

MORTIMER, PP ^{[1
]}

MCLEAN, KA ^{[1
]}

EVANS, BA ^{[1
]}

机构：

[1] W LONDON HOSP,DEPT GENITOURINARY MED,LONDON,ENGLAND

来源：

JOURNAL OF MEDICAL VIROLOGY | 1990年 / 31卷 / 02期

关键词：

EDTA anticoagulant; heparin anticoagulant; isolation frequency; T[!sub]4[!/sub] count;

D O I：

10.1002/jmv.1890310215

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human immunodeficiency virus (HIV) was cultivated directly from whole blood treated with anticoagulant by cocultivation with phytohaemagglutinin‐stimulated cord blood lymphocytes. When heparin was used as the anticoagulant, isolation rates were low (10% to 56%, depending on the patient group); but when EDTA was used, isolation rates were much higher (50% to 100%). Culture of whole blood gave results identical to those of culture of separated peripheral mononuclear cells, and in some cases virus could be isolated from as little as 10 μl of unseparated EDTA anticoagulated blood. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：161 / 164

页数：4

共 6 条

[1] MECHANISM OF INHIBITORY EFFECT OF DEXTRAN SULFATE AND HEPARIN ON REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS INVITRO [J].