STRUCTURE OF INTRACELLULAR MATURE VACCINIA VIRUS OBSERVED BY CRYOELECTRON MICROSCOPY

被引：62

作者：

DUBOCHET, J ^{[1
]}

ADRIAN, M ^{[1
]}

RICHTER, K ^{[1
]}

GARCES, J ^{[1
]}

WITTEK, R ^{[1
]}

机构：

[1] UNIV LAUSANNE,INST BIOL ANIM,CH-1015 LAUSANNE,SWITZERLAND

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 03期

关键词：

D O I：

10.1128/JVI.68.3.1935-1941.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Intracellular mature vaccinia virus, also called intracellular naked virus, and its core envelope have been observed in their native, unfixed, unstained, hydrated states by cryoelectron microscopy of vitrified samples. The virion appears as a smooth rounded rectangle of ca. 350 by 270 nm. The core seems homogeneous and is surrounded by a 30-nm-thick surface domain delimited by membranes. We show that surface tubules and most likely also the characteristic dumbbell-shaped core with the lateral bodies which are generally observed in negatively stained or conventionally embedded samples are preparation artifacts.

引用

页码：1935 / 1941

页数：7

共 23 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].

ADRIAN, M ;

DUBOCHET, J ;

LEPAULT, J ;

MCDOWALL, AW .

NATURE, 1984, 308 (5954) :32-36

[2] CRYO-ELECTRON MICROSCOPY REVEALS MACROMOLECULAR ORGANIZATION WITHIN BIOLOGICAL LIQUID-CRYSTALS SEEN IN THE POLARIZING MICROSCOPE [J].

BOOY, FP ;

FOWLER, AG .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1985, 7 (06) :327-335

[3] HAS NEGATIVE STAINING STILL A PLACE IN BIOMACROMOLECULAR ELECTRON-MICROSCOPY [J].

BREMER, A ;

HENN, C ;

ENGEL, A ;

BAUMEISTER, W ;

AEBI, U .

ULTRAMICROSCOPY, 1992, 46 (1-4) :85-111

[4]

BUIST JB, 1886, P ROY SOC EDINB, V13, P603

[5] CRYO-ELECTRON MICROSCOPY OF VITRIFIED BIOLOGICAL SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

LEPAULT, J ;

MCDOWALL, AW .

TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (04) :143-146

[6] CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

CHANG, JJ ;

HOMO, JC ;

LEPAULT, J ;

MCDOWALL, AW ;

SCHULTZ, P .

QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) :129-228

[7]

Dubochet J., 1987, CRYOTECHNIQUES BIOL, DOI 10.1007/978-3-642-72815-0_5

[8] CONTROLLED DEGRADATION OF VACCINIA VIRIONS IN VITRO - AN ELECTRON MICROSCOPIC STUDY [J].

EASTERBROOK, KB .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1966, 14 (5-6) :484-+

[9]

Fenner F., 1989, THE ORTHOPOXVIRUSES

[10]

Fenner F., 1988, HUMAN MONKEYPOX

← 1 2 3 →