DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION BY POLYMERASE CHAIN-REACTION IN A COHORT OF SERONEGATIVE INTRAVENOUS-DRUG-USERS

被引：30

作者：

FARZADEGAN, H

VLAHOV, D

SOLOMON, L

MUNOZ, A

ASTEMBORSKI, J

TAYLOR, E

BURNLEY, A

NELSON, KE

机构：

[1] Department of Epidemiology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MY

[2] Howard University School of Medicine, Washington, DC

来源：

JOURNAL OF INFECTIOUS DISEASES | 1993年 / 168卷 / 02期

关键词：

D O I：

10.1093/infdis/168.2.327

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The frequency of serologically undetected human immunodeficiency virus type 1 (HIV-1) infection among a large cohort of seronegative intravenous drug users (IVDUs) was determined by polymerase chain reaction (PCR). In total, 2159 blood specimens were obtained from 945 seronegative IVDUs; of these, 182 had 1 specimen, 339 had 2, 397 had 3, and 27 had 4 semiannual specimens. No proviral DNA was detected in 2134 (98.8%) of the samples. Specimens from 7 persons (0.3%) were reactive by PCR. Within 6 months, all 5 of these 7 who returned for follow-up visits had seroconverted. Serum from 19 persons (0.9%) were equivocal by PCR analysis, that is, single primer pair amplification; 1 person seroconverted while others subsequently remained seronegative and nonreactive by PCR. The concordance between PCR and serology was 98.6%. It is concluded that immunosilent HIV-1 infection is uncommon and that serologic screening for HIV-1 antibodies is highly sensitive in this population.

引用

页码：327 / 331

页数：5

共 31 条

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