A NUCLEAR MAGNETIC-RELAXATION STUDY OF CONFORMATIONAL-CHANGES INDUCED BY SUBSTRATE AND TEMPERATURE IN BOVINE LIVER THIOSULFATE SULFURTRANSFERASE AND YEAST HEXOKINASE

被引：14

作者：

BLICHARSKA, B

KOLOCZEK, H

WASYLEWSKI, Z

机构：

[1] JAGIELLONIAN UNIV, INST MOLEC BIOL, DEPT BIOCHEM, PL-30060 CRACOW, POLAND

[2] JAGIELLONIAN UNIV, INST PHYS, PL-30060 CRACOW, POLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1982年 / 708卷 / 03期

关键词：

D O I：

10.1016/0167-4838(82)90444-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear magnetic relaxation studies were performed on thiosulfate sulfurtransferase and hexokinase. Observation of proton spin-lattice relaxation times T1 indicates that structural transitions occur in these enzymes in the range 0-40.degree. C and that there are different temperature-dependent forms of thiosulfate sulfurtransferase and hexokinase. Thermal transitions between these forms are affected by the binding of the substrates. The results may be due to changes in the interactions between the structural domains into which the single polypeptide chains of thiosulfate sulfurtransferase and hexokinase are folded.

引用

页码：326 / 329

页数：4

共 39 条

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JOURNAL OF BIOLOGICAL CHEMISTRY, 1972, 247 (10) : 3073 - &
[32] THALLIUM-205 NUCLEAR MAGNETIC RESONANCE STUDY OF PYRUVATE KINASE AND ITS SUBSTRATES - EVIDENCE FOR A SUBSTRATE-INDUCED CONFORMATIONAL CHANGE
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JOURNAL OF BIOLOGICAL CHEMISTRY, 1971, 246 (20) : 6227 - &
[33] REVERSIBLE CONFORMATIONAL-CHANGES OF RAT-LIVER FATTY-ACID BINDING-PROTEIN FOLLOWING LIPID-BINDING - CIRCULAR DICHROIC AND NUCLEAR-MAGNETIC-RESONANCE ANALYSIS
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BIOMEDICAL RESEARCH-TOKYO, 1992, 13 (05): : 335 - 341
[34] PROTON NUCLEAR MAGNETIC-RESONANCE OF MINOR NUCLEOSIDES IN YEAST PHENYLALANINE TRANSFER RIBONUCLEIC-ACID - CONFORMATIONAL-CHANGES AS A CONSEQUENCE OF AMINOACYLATION, REMOVAL OF THE Y-BASE, AND CODON-ANTICODON INTERACTION
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[35] C-13-NUCLEAR MAGNETIC-RESONANCE STUDY OF POLYSACCHARIDE GELS - MOLECULAR ARCHITECTURE IN THE GELS CONSISTING OF FUNGAL, BRANCHED (1 -] 3)-BETA-D-GLUCANS (LENTINAN AND SCHIZOPHYLLAN) AS MANIFESTED BY CONFORMATIONAL-CHANGES INDUCED BY SODIUM-HYDROXIDE
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CARBOHYDRATE RESEARCH, 1979, 74 (SEP) : 227 - 240
[36] HYDRATION DEHYDRATION-INDUCED CONFORMATIONAL-CHANGES OF AGAROSE, AND KAPPA-CARRAGEENANS AND IOTA-CARRAGEENANS AS STUDIED BY HIGH-RESOLUTION SOLID-STATE C-13-NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
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CARBOHYDRATE RESEARCH, 1990, 199 (01) : 1 - 10
[37] SPECTROPHOTOMETRIC AND NUCLEAR MAGNETIC-RESONANCE STUDY OF TEMPERATURE-INDUCED AND PRESSURE-INDUCED CHANGES IN THE INTIMATE SOLVATION OF NEODYMIUM(III) IN N,N-DIMETHYLFORMAMIDE AND TRIMETHYLPHOSPHATE
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[38] NUCLEAR MAGNETIC-RESONANCE STUDY OF COMPLEXES OF MANGANESE(II) AND FULLY ADENYLATED GLUTAMINE-SYNTHETASE ESCHERICHIA-COLI-W - FREQUENCY, TEMPERATURE, AND SUBSTRATE DEPENDENCE OF WATER PROTON RELAXATION RATES
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BIOCHEMISTRY, 1974, 13 (16) : 3286 - 3291
[39] Aging-induced changes in microstructure and water distribution in fresh and cooked pork in relation to water-holding capacity and cooking loss - A combined confocal laser scanning microscopy (CLSM) and low-field nuclear magnetic resonance relaxation study
Straadt, Ida Krestine
Rasmussen, Marianne
Andersen, Henrik Jorgen
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MEAT SCIENCE, 2007, 75 (04) : 687 - 695

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