DEVELOPMENT AND VALIDATION OF A ROBUST SPECIFIC ENZYME MEDIATED ASSAY FOR PHENYLALANINE IN SERUM

被引：15

作者：

CAMPBELL, RS

BREARLEY, GM

VARSANI, H

MORRIS, HC

MILLIGAN, TP

HALL, SK

HAMMOND, PM

PRICE, CP

机构：

[1] UNIV LONDON LONDON HOSP, COLL MED, DEPT CLIN BIOCHEM, TURNER ST, LONDON E1 2AD, ENGLAND

[2] CTR APPL MICROBIOL & RES, DIV BIOTECHNOL, SALISBURY SP4 0JG, ENGLAND

[3] CHILDRENS HOSP, DEPT CLIN CHEM, BIRMINGHAM B16 8ET, W MIDLANDS, ENGLAND

来源：

CLINICA CHIMICA ACTA | 1992年 / 210卷 / 03期

关键词：

PHENYLALANINE; SERUM; TYROSINE; CROSS REACTIVITY; DEHYDROGENASE; COLORIMETRIC; ASSAY;

D O I：

10.1016/0009-8981(92)90205-5

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The specificity of a phenylalanine dehydrogenase, particularly with respect to cross reactivity toward tyrosine, has been shown to be pH dependent, being minimal at high pH. The dehydrogenase step has been coupled to colorimetric detection of NADH using a tetrazolium salt. The assay shows no significant cross reactivity towards a range of amino acids or drugs and correlates well with an established HPLC technique.

引用

页码：197 / 210

页数：14

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