PIGMENT-PROTEIN COMPLEXES FROM THE PHOTOSYNTHETIC MEMBRANE OF THE CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6803

被引：30

作者：

BARBATO, R ^{[1
]}

POLVERINODELAURETO, P ^{[1
]}

RIGONI, F ^{[1
]}

DEMARTINI, E ^{[1
]}

GIACOMETTI, GM ^{[1
]}

机构：

[1] CRIBI, PADUA, ITALY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 234卷 / 02期

关键词：

PHOTOSYSTEM I; PHOTOSYSTEM II; PIGMENT-PROTEIN COMPLEXES; CYANOBACTERIA;

D O I：

10.1111/j.1432-1033.1995.459_b.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photosystem I and II core complexes were resolved in a single step from the thylakoid membrane of Synechocystis sp. PCC 6803 by using a mild solubilization procedure in dodecyl P-D-maltoside and Deriphat/PAGE. For each photosystem, two green bands were obtained containing oligomeric and monomeric forms of the core complexes of either photosystem. The oligomers are likely to be trimers in the case of photosystem I and dimers for photosystem II. The absorption spectra, polypeptide and pigment composition of green bands corresponding to either photosystem I or photosystem II were identical for monomeric and oligomeric forms. The cytochrome b-559 content of photosystem II was evaluated to be one cytochrome b-559/reaction centre both in the monomeric and dimeric forms. Two new 15-kDa and 22-kDa carotenoid-binding proteins were isolated and their polypeptides purified to homogeneity.

引用

页码：459 / 465

页数：7

共 57 条

[1] ISOLATION AND CHARACTERIZATION OF THE PHOTOSYSTEM 2 REACTION-CENTER COMPLEX FROM A DOUBLE MUTANT OF CHLAMYDOMONAS-REINHARDTII [J].