IMMUNOCHEMICAL DETECTION OF URINARY 5-METHYL-2'-DEOXYCYTIDINE AS A POTENTIAL BIOLOGIC MARKER FOR LEUKEMIA

被引：23

作者：

ITOH, K ^{[1
]}

AIDA, S ^{[1
]}

ISHIWATA, S ^{[1
]}

YAMAGUCHI, T ^{[1
]}

ISHIDA, N ^{[1
]}

MIZUGAKI, M ^{[1
]}

机构：

[1] SENDAI INST MICROBIOL,SENDAI,MIYAGI,JAPAN

来源：

CLINICA CHIMICA ACTA | 1995年 / 234卷 / 1-2期

关键词：

MODIFIED NUCLEOSIDES; 5-METHYL-2'-DEOXYCYTIDINE; MONOCLONAL ANTIBODY; HUMAN URINE; MARKER FOR LEUKEMIA;

D O I：

10.1016/0009-8981(94)05986-3

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A monoclonal antibody against 5-methylcytidine was prepared and characterized. This antibody, termed AMC, was reactive with compounds that had 5-methylcytosine structure (i.e. 5-methyl-2'-deoxycytidine, 5-methylcytidine and 5-methylcytosine). AMC had the highest reactivity to 5-methyl-2'-deoxycytidine among reactive compounds and had no or very slight cross-reactivity to cytidine-related compounds and any other compounds. Analysis of immunoreactive materials in urine revealed that 5-methyl-2'-deoxycytidine rather than 5-methylcytidine was, contrary to our expectation, the major component. Then the inhibition ELISA system using AMC was established and urinary levels of 5-methyl-2'-deoxycytidine in healthy individuals and cancer patients were determined. The mean excretion levels of healthy individuals was 0.90 +/- 0.43 nmol/mu mol creatinine and the cut-off value was set at the mean + 2 S.D. of healthy individuals (1.76 nmol/mu mol creatinine). Among various types of cancer tested, elevated levels of 5-methyl-2'-deoxycytidine were detected in leukemia patients. From these results, urinary 5-methyl-2'-deoxycytidine might be applicable as a biologic marker for leukemia.

引用

页码：37 / 45

页数：9

共 17 条

[1] DNA DEMETHYLATION IN ERYTHROLEUKEMIA-CELLS [J].