EVALUATION OF RESTRICTED ACCESS MEDIA FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SULFONAMIDE ANTIBIOTIC RESIDUES IN BOVINE SERUM

Three commercially-available high-performance liquid chromatographic columns packed with restricted access media were evaluated for suitability in multi-residue direct injection analysis at the ng/ml level. The internal surface reversed-phase and shielded hydrophobic phase columns were not sufficiently retentive to separate all analytes from the tail of the matrix peak. Coelution of some of the analytes was also observed with these columns. The semi-permeable surface column was significantly more retentive and selective, providing good separation of analyte and matrix peaks. With this column, an analytical protocol requiring no organic solvents was developed for the assay of six sulfonamides at a detection limit of 25 ng/ml.