ONE-STEP TRANSFORMATION OF YEAST IN STATIONARY PHASE

被引：630

作者：

CHEN, DC

YANG, BC

KUO, TT

机构：

[1] NATL TAIWAN UNIV, GRAD INST BOT, TAIPEI 115, TAIWAN

[2] ACAD SINICA, INST BOT, TAIPEI 115, TAIWAN

[3] ACAD SINICA, INST MOL BIOL, TAIPEI, TAIWAN

来源：

CURRENT GENETICS | 1992年 / 21卷 / 01期

关键词：

YEAST; TRANSFORMATION; THIO COMPOUND; STATIONARY PHASE;

D O I：

10.1007/BF00318659

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45-degrees-C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/mu-g plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

引用

页码：83 / 84

页数：2

共 14 条

[1]

BRZOBOHATY B, 1986, J GEN MICROBIOL, V132, P3089

[2] TRANSFORMATION OF YEAST [J].