EFFECTS OF ORGANIC-SOLVENTS AND TEMPERATURE ON THE DESORPTION OF YEAST 3-PHOSPHOGLYCERATE KINASE FROM IMMUNOADSORBENT

被引：31

作者：

ANDERSSON, KK ^{[1
]}

BENYAMIN, Y ^{[1
]}

DOUZOU, P ^{[1
]}

BALNY, C ^{[1
]}

机构：

[1] ECOLE PRAT HAUTES ETUD,BIOCHIM MARINE LAB,CNRS,CTR RECH BIOCHIM MACROMOLEC,F-34033 MONTPELLIER,FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 25卷 / 04期

关键词：

D O I：

10.1016/0022-1759(79)90029-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Physicochemical parameters governing the elution of yeast 3-phosphoglycerate kinase from its immunoadsorbent were studied. Non-denaturing elution conditions were determined (alkali medium containing 50%, v/v, ethylene glycol) and the method was applied to one-step isolation of enzyme from a crude yeast preparation. © 1979.

引用

页码：375 / 381

页数：7

共 20 条

[1] ORGANIC-SOLVENTS AND TEMPERATURE EFFECTS ON DESORPTION FROM IMMUNOADSORBENTS - DNP-BSA ANTI-DNP AS A MODEL
ANDERSSON, K
BENYAMIN, Y
DOUZOU, P
BALNY, C
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1978, 23 (1-2) : 17 - 21
[2] AFFINITY CHROMATOGRAPHY AT SUB-ZERO TEMPERATURES - MODEL STUDY WITH PORCINE PANCREATIC ELASTASE
BALNY, C
LEDOUCEN, C
DOUZOU, P
BIETH, JG
[J]. JOURNAL OF CHROMATOGRAPHY, 1979, 168 (01): : 133 - 138
[3] UBER EIN PHOSPHATUBERTRAGENDES GARUNGSFERMENT
BUCHER, T
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1947, 1 (04) : 292 - 314
[4] DEUTSCH HF, 1967, METHODS IMMUNOLOGY I, V1, P315
[5] Douzou P, 1978, Adv Protein Chem, V32, P77, DOI 10.1016/S0065-3233(08)60575-6
[6] GORNALL AG, 1949, J BIOL CHEM, V177, P751
[7] HILL R J, 1972, Journal of Immunological Methods, V1, P231, DOI 10.1016/0022-1759(72)90002-6
[8] SPECTROPHOTOMETRIC PH TITRATIONS AND NITRATION WITH TETRANITROMETHANE OF TYROSYL RESIDUES IN YEAST PHOSPHOGLYCERATE KINASE
HJELMGREN, T
ARVIDSSON, L
LARSSONRAZNIKIEWICZ, M
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 445 (02) : 342 - 349
[9] HUIBONHO.G, 1973, J BIOL CHEM, V248, P4649
[10] KRIETSCH W K G, 1970, European Journal of Biochemistry, V17, P568, DOI 10.1111/j.1432-1033.1970.tb01202.x

← 1 2 →