EFFECT OF PHOTOOXIDATION ON HUMAN CAERULOPLASMIN

被引：20
作者：
BANNISTER, WH
WOOD, EJ
机构：
[1] Department of Physiology and Biochemistry, The Royal University of Malta, Msida
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 01期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00758.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human caeruloplasmin showed a rapid loss of p‐phenylene‐diamine oxidase activity when subjected to photooxidation in the presence of methylene blue. Major conformational changes did not occur. The kinetic characteristics of the loss of phenylene‐diamine oxidase activity were comparable to those of the destruction of histidine residues, and the relative extent of the two processes indicated a one‐to‐one correspondence between them. Copper was lost at a slower rate than that of destruction of histidine residues. It is suggested that the site responsible for the oxidase activity of caeruloplasmin may contain an essential histidine residue and that, although histidine residues may also be involved in copper binding, they are not exclusive copper ligands. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：179 / +
页数：1
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