RECIPROCAL CONTROL OF INFLAMMATORY CYTOKINES, IL-1 AND IL-6, AND BETA-AMYLOID PRODUCTION IN CULTURES

被引：122

作者：

DELBO, R ^{[1
]}

ANGERETTI, N ^{[1
]}

LUCCA, E ^{[1
]}

DESIMONI, MG ^{[1
]}

FORLONI, G ^{[1
]}

机构：

[1] IST RIC FARMACOL MARIO NEGRI,I-20157 MILAN,ITALY

来源：

NEUROSCIENCE LETTERS | 1995年 / 188卷 / 01期

关键词：

ALZHEIMERS DISEASE; BETA-AMYLOID; ASTROCYTES; GENE EXPRESSION; CYTOKINES;

D O I：

10.1016/0304-3940(95)11384-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To investigate the role of IL-6 in the pathogenesis of Alzheimer's disease (AD) its effect on amyloid precursor protein (APP) mRNA expression was evaluated. The levels of APP mRNA were determined by Northern blot analysis in primary cultured rat cortical neurons and glial cells exposed to IL-6 (50-200 ng/ml). The cytokine increased neuronal APP mRNA expression about 100% at the highest dose after 6 h of exposure. APP mRNA expression was unaffected in astroglial cells exposed to IL-6. Since IL-1 beta also increased neuronal APP mRNA, the combination of IL-1 beta and IL-6 was tested. The effects were partially additive. The ability of beta-amyloid fragment 25-35 to induce IL-1 or IL-6 mRNA was also investigated in astroglial cells. IL-1 beta mRNA was strongly induced by beta 25-35 (25-100 mu M) while the expression of IL-6 mRNA remaining unchanged. The results suggest roles for both IL-1 and IL-6 in the neuronal mechanisms related to beta-amyloid protein deposition in AD.

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收藏

页码：70 / 74

页数：5

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