High-Frequency Transformation of Arabidopsis thaliana by Agrobacterium tumefaciens

被引：71

作者：

Clarke, Michael C. ^{[1
]}

Wei, Wenbin ^{[1
]}

Lindsey, Keith ^{[1
]}

机构：

[1] Univ Leicester, Leicester Bioctr, Leicester LE1 7RH, Leics, England

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 02期

关键词：

Agrobacterium tumefaciens-mediated transformation; Arabidopsis thaliana; silver thiosulphate;

D O I：

10.1007/BF02668345

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Incorporation of 5 mg/L silver thiosulphate into media for seed germination and callus induction, as used in the transformation protocol originally described by Valvekens et al. (1988), was found to increase the frequency of regeneration of transformants of Arabidopsis thaliana ecotypes C24 and Landsberg erecta by at least 10- to 100-fold. Other factors, such as density of the bacterial inoculation culture, density of the root explants and duration of bacteria-plant cocultivation period, were also found to influence the efficiency of recovery of transformants.

引用

页码：178 / 189

页数：12

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