A MURINE CDC25/RAS-GRF-RELATED PROTEIN IMPLICATED IN RAS REGULATION

被引：27
作者：
CHEN, LP
ZHANG, LJ
GREER, P
TUNG, PS
MORAN, MF
机构：
[1] UNIV TORONTO, BANTING & BEST DEPT MED RES, TORONTO M5G 1L6, ONTARIO, CANADA
[2] UNIV TORONTO, DEPT MOLEC & MED GENET, TORONTO M5G 1L6, ONTARIO, CANADA
[3] QUEENS UNIV, CANC RES LABS, KINGSTON K7L 3N6, ONTARIO, CANADA
来源：
DEVELOPMENTAL GENETICS | 1993年 / 14卷 / 05期
关键词：
RAS; CDC25; GUANINE NUCLEOTIDE RELEASE FACTOR; SIGNAL TRANSDUCTION; EMBRYONIC STEM CELL;
D O I：
10.1002/dvg.1020140503
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A partial cDNA encoding a novel putative p2, ras guanine nucleotide release-inducing factor (GRF), GRF2, was amplified from murine embryonic stem cells. The presumptive catalytic region of GRF2 is related to the yeast Ras GRF encoded by CDC25. GRF2 is 80% identical to murine CDC25Mm/ras-GRF, but is more similar to yeast CDC25 than to other ras GRFs related to the Drosophila son of sevenless gene product. A 9-kb GRF2 messenger RNA was highly expressed in brain, but GRF2-specific antibodies recognized apparent GRF2 proteins in various mouse tissues in addition to brain. Thus GRF2 represents a novel widely-expressed protein that is highly related to CDC25Mm/ras-GRF, at least in its catalytic domain. Both GRF2 and CDC25Mm/ras-GRF are expressed in murine embryonic stem cells, suggesting that different Ras activators may regulate ras-dependent proliferation and differentiation in early mouse development. (C) 1993 Wiley-Liss, Inc.
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页码：339 / 346
页数：8
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