CHANGES IN CONTENT OF GLUCOSINOLATES AND ITS ACCUMULATION IN SILIQUAE AND SEEDS OF OILRAPE (BRASSICA-NAPUS L)

Rape plants were labelled by applying ((NH4)2 (SO4))-S-35 to soil. Changes in content of S-35 in various constituents in pods and grains were determined during siliquae development to exploit formation and accumulation of glucosinolates in oilseed rape. Content of S-35 in glucosinolates expressed as mu-mol S/g.d.w. and its relative amounts in extractable forms in young siliquae were in constant level within one week after flowering, but either absolute content or relative content of S-35 in glucosinolates increased largely by two weeks after flowering, thereafter the distribution of extractable S-35 in glucosinolates of siliquae and grains increased linearly as proceeding of its development, S-35 in extracts of grains almost was in form of glucosinolates after 8 weeks from flowering. Amounts of both S-35 in glucosinolates and dry matter per pod increased linearly with time after flowering. According to the changes of amounts of S-35 in other constituents per pod, it could be supposed that glucosinolates accumulated in seeds might be transported from other organs together with nutrients.