FREQUENT AMPLIFICATION OF THE CYCLIN-E GENE IN HUMAN GASTRIC CARCINOMAS

被引：133

作者：

AKAMA, Y ^{[1
]}

YASUI, W ^{[1
]}

YOKOZAKI, H ^{[1
]}

KUNIYASU, H ^{[1
]}

KITAHARA, K ^{[1
]}

ISHIKAWA, T ^{[1
]}

TAHARA, E ^{[1
]}

机构：

[1] HIROSHIMA UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 2,MINAMI KU,HIROSHIMA 734,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1995年 / 86卷 / 07期

关键词：

CELL CYCLE; G1; CYCLINS; G1/S TRANSITION;

D O I：

10.1111/j.1349-7006.1995.tb02442.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We searched for genetic alterations of the cyclin D1 and cyclin E genes in 45 human gastric carcinoma tissues. Expression of cyclin E mRNA and protein was also analyzed in eight of them by Northern and Western blots and immunohistochemical staining. The cyclin E gene was amplified 3-10 fold in seven gastric cancer tissues (15.6%), of which six were advanced gastric cancers. ALI of the cases with the cyclin E gene amplification displayed lymph node metastasis. Moreover, the case with the gene amplification overexpressed the cyclin E mRNA and protein. One of eight gastric cancer cell lines, MKN-7, shared the cyclin E gene amplification, and all of the gastric cancer cell lines expressed high levels of the cyclin E mRNA and protein even without gene amplification. Amplification of the cyclin D1 gene was not observed in any of the gastric carcinoma tissues or gastric carcinoma cell lines. These results suggest that the gene amplification and overexpression of cyclin E play an important role in the abnormal growth and progression of gastric carcinoma.

引用

页码：617 / 621

页数：5

共 30 条

[1]

BUKLEY MF, 1993, ONCOGENE, V8, P2127

[2]

DOU QP, 1993, CANCER RES, V53, P1493

[3] ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE [J].

DULIC, V ;

LEES, E ;

REED, SI .

SCIENCE, 1992, 257 (5078) :1958-1961

[4]

GILLETT C, 1994, CANCER RES, V54, P1812

[5] K-SAM, AN AMPLIFIED GENE IN STOMACH-CANCER, IS A MEMBER OF THE HEPARIN-BINDING GROWTH-FACTOR RECEPTOR GENES [J].

HATTORI, Y ;

ODAGIRI, H ;

NAKATANI, H ;

MIYAGAWA, K ;

NAITO, K ;

SAKAMOTO, H ;

KATOH, O ;

YOSHIDA, T ;

SUGIMURA, T ;

TERADA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5983-5987

[6] CYCLINS AND CANCER .2. CYCLIN-D AND CDK INHIBITORS COME OF AGE [J].

HUNTER, T ;

PINES, J .

CELL, 1994, 79 (04) :573-582

[7]

JIANG W, 1992, CANCER RES, V52, P2980

[8]

KAMEDA T, 1990, CANCER RES, V50, P8002

[9]

KEYOMARSI K, 1994, CANCER RES, V54, P380

[10] REDUNDANT CYCLIN OVEREXPRESSION AND GENE AMPLIFICATION IN BREAST-CANCER CELLS [J].

KEYOMARSI, K ;

PARDEE, AB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (03) :1112-1116

← 1 2 3 →