Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes

被引：9

作者：

Temmerman, Liesbet ^{[2
,3
]}

De Livera, Alysha M. ^{[1
]}

Bowne, Jairus B. ^{[1
]}

Sheedy, John R. ^{[1
]}

Callahan, Damien L. ^{[2
]}

Nahid, Amsha ^{[1
]}

De Souza, David P. ^{[1
]}

Schoofs, Liliane ^{[3
]}

Tull, Dedreia L. ^{[1
]}

McConville, Malcolm J. ^{[1
]}

Roessner, Ute ^{[2
,6
]}

Wentworth, John M. ^{[4
,5
]}

机构：

[1] Metabolom Australia, Inst Bio21, Melbourne, Vic 3052, Australia

[2] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia

[3] Katholieke Univ Leuven, Funct Genom & Proteom, B-3000 Louvain, Belgium

[4] Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3052, Australia

[5] Monash Univ, Ctr Obest Res & Educ, Clayton, Vic 3004, Australia

[6] Univ Melbourne, Sch Bot, Australian Ctr Plant Funct Genom, Melbourne, Vic 3010, Australia

来源：

JOURNAL OF DIABETES & METABOLISM | 2013年 / 4卷

关键词：

Metabolomics; Urine; Hyperglycemia; Hyperglycaemia; Diabetes; NMR; GC; LC; MS; Biomarker;

D O I：

10.4172/2155-6156.S6-002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Hyperglycemia causes diabetic nephropathy, a condition for which there are no specific diagnostic markers that predict progression to renal failure. Here we describe a multiplatform metabolomic analysis of urine from individuals with type 2 diabetes, collected before and immediately following experimental hyperglycemia. We used targeted nuclear magnetic resonance spectroscopy (NMR), liquid chromatography - mass spectrometry (LC-MS) and gas chromatography - MS (GC-MS) to identify markers of hyperglycemia. Following optimization of data normalisation and statistical analysis, we identified a reproducible NMR and LC-MS based urine signature of hyperglycemia. Significant increases of alanine, alloisoleucine, isoleucine, leucine, N-isovaleroylglycine, valine, choline, lactate and taurine and decreases of arginine, gamma-aminobutyric acid, hippurate, suberate and N-acetylglutamate were observed. GC-MS analysis identified a number of metabolites differentially present in post-glucose versus baseline urine, but these could not be identified using current metabolite libraries. This analysis is an important first step towards identifying biomarkers of early-stage diabetic nephropathy.

引用

页数：7

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