PURIFICATION AND CHARACTERIZATION OF AN ENDOPOLYGALACTURONASE FROM PHYSALOSPORA-PIRICOLA

被引：7

作者：

MIYAIRI, K

MATSUE, T

KAGAWA, O

KUTSUZAWA, T

OKUNO, T

机构：

[1] Department of Bioresources Science, Faculty of Agriculture, Hirosaki University, Hirosaki-shi

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 10期

关键词：

D O I：

10.1271/bbb.58.1909

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An endopolygalacturonase from Physalospora piricola was purified to apparent homogeneity by column chromatographies. The molecular weight was estimated to be 38,000 by SDS-PAGE, and the isoelectric point was around pH 7.6. The amino acid composition was similar to those of endo PGs from other fungi. The enzyme was most active at pH 4.9 and stable from pH 5 to 7. Oligogalacturonic acids with a degree of polymerization higher than three were readily hydrolyzed by the enzyme, but trigalacturonic acid only slightly.

引用

页码：1909 / 1910

页数：2

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