MIXED ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF URIDINE 5'-DIPHOSPHO-ALPHA-D-GLUCURONIC ACID AND ITS HYDROLYSIS PRODUCTS

被引：3

作者：

BEDFORD, CT ^{[1
]}

HICKMAN, AD ^{[1
]}

LOGAN, CJ ^{[1
]}

机构：

[1] SHELL RES LTD,SITTINGBOURNE BIOSCI RES CTR,SITTINGBOURNE ME9 8AG,KENT,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 695卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)01172-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although conventional ion-exchange HPLC and ion-pair reversed-phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5'-diphospho-alpha-D-glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5'-diphospho-alpha-D-glucuronic acid (alpha-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining alpha-UDPGA and its hydrolysis products, UDP, UMP and cUMP.

引用

页码：123 / 127

页数：5

共 6 条

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