SECRETION OF HUMAN HEPATITIS-B VIRUS IS INHIBITED BY THE IMINO SUGAR N-BUTYLDEOXYNOJIRIMYCIN

被引：170
作者：
BLOCK, TM
LU, XY
PLATT, FM
FOSTER, GR
GERLICH, WH
BLUMBERG, BS
DWEK, RA
机构：
[1] UNIV OXFORD,INST GLYCOBIOL,OXFORD OX1 3QU,OXON,ENGLAND
[2] JUSTUS LIEBIG UNIV,INST MED VIROL,W-6300 GIESSEN,GERMANY
[3] IMPERIAL COLL SCI TECHNOL & MED,ST MARYS HOSP,DEPT MED,LONDON W2 1PG,ENGLAND
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 06期
关键词：
ANTIVIRALS; GLYCOSYLATION;
D O I：
10.1073/pnas.91.6.2235
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The imino sugar N-butyldeoxynojirimycin (NBDNJ) is a potent inhibitor of the oligosaccharide-trimming enzyme alpha-glucosidase I. Hepatitis B virus (HBV) contains three surface proteins (HBs proteins) of different sizes that are singly or doubly N-glycosylated and are essential for the formation of infectious virus. Therefore, the replication and secretion of HBV in the human hepatoma cell line HepG2 were studied in the presence of NBDNJ. In the stably HBV-transfected HepG 2.2.15 cells and in HBV-infected HepG2 cells, NBDNJ suppressed secretion of HBV particles and caused intracellular retention of HBV DNA. The secretion of subviral particles was less affected. These data suggest that inhibitors of oligosaccharide trimming may be useful for antiviral therapy of hepatitis B and for the study of the intracellular transport of the viral glycoproteins.
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页码：2235 / 2239
页数：5
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