INHIBITION OF HEMATOPOIETIC DEVELOPMENT FROM EMBRYONIC STEM-CELLS BY ANTISENSE VAV RNA

被引：52
|
作者：
WULF, GM [1 ]
ADRA, CN [1 ]
LIM, B [1 ]
机构：
[1] HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DEPT MED,DIV HEMATOL & ONCOL,BOSTON,MA 02215
来源：
EMBO JOURNAL | 1993年 / 12卷 / 13期
关键词：
ANTISENSE RNA; EMBRYONIC STEM CELLS; HEMATOPOIESIS; VAV PROTOONCOGENE;
D O I：
10.1002/j.1460-2075.1993.tb06200.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The vav proto-oncogene is universally and specifically expressed in hematopoietic cells. vav contains a unique array of motifs allowing the protein to function as a signal transducer and possibly as a transcription factor. Under certain in vitro culture conditions murine embryonic stem cells develop into colonies containing multiple hematopoietic lineages. In embryonic stem cell lines, constitutively expressing high levels of antisense vav transcripts through a stably integrated transgene, differentiation into hematopoietic cells is disrupted. This observation presents the first evidence that vav has a critical role in the development of hematopoietic cells from totipotent cells.
引用
收藏
页码：5065 / 5074
页数：10
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