MOLECULAR-CLONING AND CHARACTERIZATION OF AN INTERFERON INDUCED HUMAN CDNA WITH SEQUENCE HOMOLOGY TO A MAMMALIAN PEPTIDE-CHAIN RELEASE FACTOR

被引：35

作者：

BUWITT, U ^{[1
]}

FLOHR, T ^{[1
]}

BOTTGER, EC ^{[1
]}

机构：

[1] MED HSCH HANNOVER, INST MED MIKROBIOL, KONSTANTY GUTSCHOW STR 8, W-3000 HANNOVER 61, GERMANY

来源：

EMBO JOURNAL | 1992年 / 11卷 / 02期

关键词：

HUMAN CDNA; INTERFERON INDUCTION; MOLECULAR CLONING; RELEASE FACTOR;

D O I：

10.1002/j.1460-2075.1992.tb05079.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report the molecular cloning of several related human cDNAs from which a full-length sequence can be determined. The cDNAs encode a 2.8 kb mRNA that is strongly induced by interferon (IFN)-gamma and the expression of which is not cell-restricted but observed in fibroblasts, macrophages and epithelial cells. The deduced amino acid sequence predicts a protein of 471 amino acids with high sequence similarity to a previously identified rabbit peptide chain release factor. Functional studies to demonstrate release factor activity showed that the protein encoded by this cDNA inhibited the readthrough activity of a yeast UGA suppressor tRNA in an in vitro translation system. The identification of this novel cDNA implies that translational control by IFN induced proteins may not be restricted to the initial steps of protein synthesis but may also act by regulation of peptide chain termination.

引用

页码：489 / 496

页数：8

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