PREPARATIVE CELL ELECTROPHORESIS WITH D2O AS A STABILIZING AGENT

被引：14

作者：

BRONSON, PM

VANOSS, CJ

机构：

[1] Immunochemistry Laboratory, Department of Microbiology, State University of New York at Buffalo, Buffalo

来源：

PREPARATIVE BIOCHEMISTRY | 1979年 / 9卷 / 01期

关键词：

D O I：

10.1080/00327487908061672

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The method of ascending preparative cell electrophoresis in a non-stabilized vertical column can be greatly improved by layering the cells on a starting cushion of buffer prepared in D20 and by stabilizing the liquid column above it with a D2O gradient. D2O/H2O mixtures have no apparent biochemical effects on the cells, and their physicochemical effects are short-lived and reversible. It was possible, by this method, to separate a mixture of glutaraldehyde-fixed erythrocytes of three species (rabbit, horse and chicken) into three distinct zones after 15 minutes of electrophoresis. It was also possible to obtain an enriched human lymphocyte fraction containing 96% T-cells, after 1 hour of electrophoresis. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：61 / 70

页数：10

共 16 条

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