CARBACHOL MIMICS PHORBOL ESTERS IN ITS ABILITY TO ENHANCE CYCLIC-GMP PRODUCTION BY STA, THE HEAT-STABLE TOXIN OF ESCHERICHIA-COLI

被引：16
作者：
CRANE, JK
BURRELL, LL
WEIKEL, CS
GUERRANT, RL
机构：
[1] UNIV VIRGINIA,DIV GEOG MED,CHARLOTTESVILLE,VA 22908
[2] JOHNS HOPKINS UNIV,SCH MED,DIV INFECT DIS,BALTIMORE,MD 21205
[3] JOHNS HOPKINS UNIV,SCH MED,DIV GASTROENTEROL,BALTIMORE,MD 21205
来源：
FEBS LETTERS | 1990年 / 274卷 / 1-2期
关键词：
Carbachol: Protein kinase C; Escherichia coli: Guanosine 3'; 5'-cyclic monophosphate: Phorbol ester; Heat-stable enterotoxin; Muscarinic receptor;
D O I：
10.1016/0014-5793(90)81363-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
STa, the heat-stable enterotoxin of Escherichiu coli, stimulates membrane-bound guanylate cyclase in enterocytes, elevates cyclic GMP. and results in intestinal secretion of ions and fluid. Using the T84 colon carcinoma cell line as a model, Weikel et al. reported that phorbol esters enhance STa-stimulated cyclic GMP production by 60 140% [(1990) Infect. Immun. 58, 1402-1407]. In the present report we demonstrate that the acetylcholinc analog carbachol enhanced toxin-stimulated cyclic GMP accumulation in intact T84 cells by 50-100% and that this effect was blocked by 10 μm atropine and 10μM sphingosine. Pertussis toxin treatment of the T84 cells did not affect the subsequent response to carbachol. Carbachol. which elevates intracellular calcium in these cells, may act through protein kinase C to enhance cyclic GMP production. © 1990.
引用
收藏
页码：199 / 202
页数：4
相关论文
共 27 条
[21]   DIFFERENTIAL REGULATION OF PI HYDROLYSIS AND ADENYLYL CYCLASE BY MUSCARINIC RECEPTOR SUBTYPES [J].
PERALTA, EG ;
ASHKENAZI, A ;
WINSLOW, JW ;
RAMACHANDRAN, J ;
CAPON, DJ .
NATURE, 1988, 334 (6181) :434-437
[22]   STRUCTURE AND REGULATION OF MUSCARINIC RECEPTORS [J].
SCHIMERLIK, MI .
ANNUAL REVIEW OF PHYSIOLOGY, 1989, 51 :217-227
[23]   PHORBOL ESTERS INCREASE ADENYLATE-CYCLASE ACTIVITY AND STABILITY IN PITUITARY MEMBRANES [J].
SUMMERS, ST ;
WALKER, JM ;
SANDO, JJ ;
CRONIN, MJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (01) :16-24
[24]  
VAROL FG, 1989, J PHARMACOL EXP THER, V249, P11
[25]  
WALDMAN SA, 1987, PHARMACOL REV, V39, P163
[26]   INTESTINAL RECEPTOR FOR HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI IS TIGHTLY COUPLED TO A NOVEL FORM OF PARTICULATE GUANYLATE-CYCLASE [J].
WALDMAN, SA ;
KUNO, T ;
KAMISAKI, Y ;
CHANG, LY ;
GARIEPY, J ;
OHANLEY, P ;
SCHOOLNIK, G ;
MURAD, F .
INFECTION AND IMMUNITY, 1986, 51 (01) :320-326
[27]   PHORBOL ESTERS ENHANCE THE CYCLIC-GMP RESPONSE OF T84-CELLS TO THE HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI (STA) [J].
WEIKEL, CS ;
SPANN, CL ;
CHAMBERS, CP ;
CRANE, JK ;
LINDEN, J ;
HEWLETT, EL .
INFECTION AND IMMUNITY, 1990, 58 (05) :1402-1407
123