2-DEOXY-2-FLUORO-L-FUCOSE AND ITS EFFECT ON L-[1-C-14] FUCOSE UTILIZATION IN MAMMALIAN-CELLS

2-Deoxy-2-fluoro-L-fucose has been obtained as a chromatographically pure compound from 3,4-O-acetyl-L-fucal and its structure established by NMR measurements. Mouse fibroblasts (TAL/N P and TAL/N B) in culture incorporate L-[1-14C-] fucose into glycoproteins of trypsinates and of cell components. The labelling is competitively and progressively diminished when 2-deoxy-2-fluoro-L-fucose or L-fucose is added to the medium in the range 0.1mM to 10mM. Evidence suggests that the fluorosugar is incorporated into glycoprotein fractions. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.