ULTRASTRUCTURAL-LOCALIZATION OF CALCIUM IN ISCHEMIC HIPPOCAMPAL SLICES - THE INFLUENCE OF ADENOSINE AND THEOPHYLLINE

被引：30

作者：

DUX, E ^{[1
]}

SCHUBERT, P ^{[1
]}

KREUTZBERG, GW ^{[1
]}

机构：

[1] MAX PLANCK INST PSYCHIAT,KLOPFERSPITZ 18A,W-8033 MARTINSRIED,GERMANY

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 1992年 / 12卷 / 03期

关键词：

HIPPOCAMPAL SLICE; ISCHEMIA; CALCIUM; MITOCHONDRIA; ADENOSINE; THEOPHYLLINE;

D O I：

10.1038/jcbfm.1992.71

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Calcium was localized ultrastructurally with the use of the modified oxalate-pyroantimonate reaction in the CA1 region of rat hippocampal slices. Ten-minute ischemia (incubation with anoxic and glucose-free medium) followed by 30 min reoxygenation resulted in mitochondrial calcium sequestration and ultrastructural damage. The addition of the adenosine receptor antagonist, theophylline, worsened the ischemia-induced morphological changes and particularly exaggerated the Ca2+ loading in the postsynaptic dendrites. In contrast, adenosine protected against ischemia-induced changes. The results suggest that adenosine exerts its neuroprotective action largely by maintaining intracellular calcium-homeostasis.

引用

页码：520 / 524

页数：5

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