INFLUENCE OF FATTY-ACID AND TIME OF FOCUSING ON ISOELECTRIC-FOCUSING OF HUMAN-PLASMA ALBUMIN

被引：41

作者：

BASU, SP

RAO, SN

HARTSUCK, JA

机构：

[1] UNIV OKLAHOMA,HLTH SCI CTR,DEPT BIOCHEM & MOLEC BIOL,OKLAHOMA CITY,OK 73104

[2] UNIV OKLAHOMA,HLTH SCI CTR,DEPT PHYSIOL & BIOPHYS,OKLAHOMA CITY,OK 73104

[3] OKLAHOMA MED RES FDN,PROT STUDIES LAB,OKLAHOMA CITY,OK 73104

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 533卷 / 01期

关键词：

D O I：

10.1016/0005-2795(78)90548-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the isoelectric focusing of human plasma albumin, two major peaks of pI 4.8 and 5.6 are observed. As fatty acids are removed from the albumin either by defatting before the focusing experiment or gradually during the focusing experiment, the pI 4.8 peak diminishes and the pI 5.6 peak increases. The interpretation of this effect is confirmed by fatty acid analysis before and after focusing. Alkylation of the free sulfhydryl group changes the focusing position of the defatted peak to pI 5.7. Otherwise the isoelectric focusing pattern of human albumin is unaffected by the status of the free sulfhydryl group or by the ampholine concentration. © 1978.

引用

页码：66 / 73

页数：8

共 20 条

[1]

BASU SP, 1977, THESIS U OKLAHOMA

[2]

CHEN RF, 1967, J BIOL CHEM, V242, P173

[3] INSTABILITY OF PH GRADIENTS FORMED BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE-GEL [J].