Kinetics of the Individual and Joint Oxidation of Dihydroquercetin and Potassium Ferrocyanide in the Presence of Horseradish Peroxidase

被引：1

作者：

Rogozhin, V. V. ^{[1
]}

Peretolchin, D., V ^{[1
]}

机构：

[1] Yakutsk State Agr Acad, Sakha Republic, Russia

来源：

MOSCOW UNIVERSITY CHEMISTRY BULLETIN | 2010年 / 65卷 / 06期

关键词：

horseradish peroxidase; dihydroquercetin; potassium; ferrocyanide; enzyme catalysis;

D O I：

10.3103/S0027131410060118

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The study-state kinetics of individual and cooxidation of ferrocyanide and dihydroquercetin catalyzed by horseradish peroxidase is studied. It is shown that ferrocyanide and dihydroquercetin are slowly oxidized substrata peroxidase. In an interval of pH 4.5-8.0, sizes k(cat) and K-m for these substrata peroxidase are certain. In reactions of cooxidation substrata, it was revealed that, at pH 4.5-7.0, dihydroquercetin accelerated oxidation ferrocyanide. At pH 7.5 oxidation only dihydroquercetin was observed. Mechanisms of reactions of combined oxidation ferrocyanide and dihydroquercetin are offered.

引用

页码：392 / 396

页数：5

共 15 条

[1]

Chance B, 1943, J BIOL CHEM, V151, P553

[2] THE PROPERTIES OF THE ENZYME-SUBSTRATE COMPOUNDS OF HORSE-RADISH AND LACTO-PEROXIDASE [J].