COMPARISON OF THE PHOSPHATE-REPRESSIBLE AND PHOSPHATE-CONSTITUTIVE ACID-PHOSPHATASES OF YARROWIA-LIPOLYTICA
被引:1
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作者:
GALABOVA, DN
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GALABOVA, DN
VASILEVATONKOVA, ES
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VASILEVATONKOVA, ES
BALASHEVA, MA
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BALASHEVA, MA
机构:
来源:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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1994年
/
10卷
/
04期
关键词:
ACID PHOSPHATASE;
YARROWIA-LIPOLYTICA;
D O I:
10.1007/BF00144480
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
The phosphate-repressible acid phosphatase from Yarrowia lipolytica cells was more thermostable but more sensitive to urea denaturation and to the effect of Triton X-100 than the constitutive counterpart. The K. values of the repressible and constitutive enzymes for p-nitrophenyl phosphate were 3.6 mM and 7.4 mM, respectively. They are judged to be distinct proteins.